000			02862cam a2200373 a 4500
001			8907
003			BD-DhEWU
005			20190508133234.0
008			190508s2016 njua g b 001 0 eng d
010			_a 2016440109
020			_a9781118873656 (cloth)
035			_a(OCoLC) 952202132
040			_aDLC _beng _erda _cDLC _dBD-DhEWU
041			_aeng
050	0	0	_aQH585.2 _b.F74 2016
082	0	0	_a571.538 _223 _bFRC 2016
100	1		_aFreshney, R. Ian, _924386
245	1	0	_aCulture of animal cells : _ba manual of basic technique and specialized applications / _cR. Ian Freshney, reviewing editors, Amanda Capes-Davis, Carl Gregory, Stefan Przyborski.
250			_a7th ed.
260			_aNew Jersey: _bWiley Blackwell, _cc2016
300			_axxxviii, 684 p. : _billustrations (some color) ; _c29 cm.
504			_aIncludes bibliographical references and index.
505			_aList of Figures, xix List of Color Plates, xxiii List of Tables, xxv List of Protocols, xxvii List of Minireviews, xxix Preface and Acknlowledgments, xxxi Abbreviations, xxxiii About the Companion Website xxxviii 1. Introduction, 1 2. Biology of Cultured Cells, 19 3. Laboratory Design and Layout, 37 4. Equipment and Materials, 51 5. Aseptic Technique, 73 6. Safety, Bioethics, and Validation, 89 7. Culture Vessels and Substrates, 111 8. Defined Media and Supplements, 125 9. Serum-Free Media, 149 10. Preparation and Sterilization, 173 10. Supplementary Material* 11. Primary Culture, 207 11. Supplementary Material* 12. Subculture and Cell Lines, 235 13. Authentication and Validation, 259 14. Microbial Contamination, 289 15. Cryopreservation and Banking, 307 16. Cloning and Selection, 327 16. Supplementary Material* 17. Cell Sorting, 347 18. Cell Line Characterization, 359 19. Differentiation, 383 20. Three ]Dimensional Culture 401 21. Scale ]up and Automation, 427 22. Senescence, Immortalization, and Transformation, 455 23. Quantitation, 483 23 Supplementary Material* 24. Cytotoxicity, 513 24. Supplementary Material* 25. Culture of Specific Cell Types, 531 25. Supplementary Material* 26. Stem Cells, 571 26. Supplementary Material* 27. Training Programs, 591 28. Problem Solving 627 29. In Conclusion, 651 Supplementary Material* Appendix III: Sources of Equipment and Material Appendix IV: Suppliers and Other Resource
526			_aGEB
590			_aAbdul Latif
650		0	_aTissue culture _vLaboratory manuals. _92604
650		0	_aCell culture _vLaboratory manuals. _924387
700	1		_aCapes-Davis, Amanda, _eeditor. _924388
700	1		_aGregory, Carl, _eeditor. _924389
700	1		_aPrzyborski, Stefan, _eeditor. _924390
856	4	2	_3WorldCat Details _uhttps://www.worldcat.org/title/culture-of-animal-cells-a-manual-of-basic-technique-and-specialized-applications/oclc/952202132&referer=brief_results
942			_2ddc _cTEXT
999			_c8907 _d8907
999			_c8907 _d8907