000 02510nam a2200325 i 4500
001 7270
003 BD-DhEWU
005 20180113103535.0
008 130925s2013 ne a g bf 001 0 eng d
010 _a 2012540035
020 _a9780124159204 (pbk.)
035 _a(OCoLC) 798825921
040 _aDLC
_beng
_cDLC
_erda
_dBD-DhEWU
041 _aeng
050 0 0 _aQK725
_b.S64 2013
082 0 4 _a571.2 SMP
_b2013
100 1 _aSmith, Roberta H.
_92603
245 1 0 _aPlant tissue culture :
_btechniques and experiments /
_cRoberta H. Smith, Emeritus Professor, Department of Horticulture, Vegetable Crops Improvement Center, Texas A&M University, College Station, Texas.
250 _aThird edition.
260 _aAmsterdam :
_bAcademic Press,
_c[2013]
300 _axvi, 188 pages :
_billustrations ;
_c23 cm
520 _a Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature .
526 _aGEB
590 _aTahur Ahmed
650 0 _aPlant tissue culture
_vLaboratory manuals.
_2SLSH
_92604
856 _3OCLC
_uhttp://www.worldcat.org/title/plant-tissue-culture-techniques-and-experiments/oclc/798825921&referer=brief_results
856 4 2 _3E-book Fulltext
_uhttp://lib.ewubd.edu/ebook/7270
942 _2ddc
_cTEXT
_01
999 _c7270
_d7270
999 _c7270
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